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Golgi anticorps

Cet anticorps Lapin Monoclonal détecte spécifiquement dans IHC (p). Il présente une réactivité envers Humain.
N° du produit ABIN6654321

Aperçu rapide pour Golgi anticorps (ABIN6654321)

Antigène

Golgi

Reactivité

Humain

Hôte

  • 5
  • 1
Lapin

Clonalité

  • 6
Monoclonal

Conjugué

  • 6
Inconjugué

Application

  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

GLG1-2829R
  • Purification

    Purified

    Pureté

    Protein A affinity chromatography

    Immunogène

    SU-DHL-1 large cell lymphoma cells were used as the immunogen for this recombinant Golgi antibody.

    Isotype

    IgG kappa
  • Indications d'application

    The optimal dilution of the recombinant Golgi antibody for each application should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    Restrictions

    For Research Use only
  • Buffer

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store the recombinant Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène

    Golgi

    Sujet

    This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
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